Catalog: YP0691
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Main Information
Target
Cyclin B1 Phospho Ser147
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ELISA
MW
60kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):AFsDV
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
60kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser147. AA range:121-170
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Specificity:
Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):AFsDV
show all
Gene Name:
CCNB1 CCNB
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Protein Name:
G2/mitotic-specific cyclin-B1
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Other Name:
CCNB1 ;
CCNB ;
G2/mitotic-specific cyclin-B1
CCNB ;
G2/mitotic-specific cyclin-B1
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Database Link:
Background:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008],
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Function:
developmental stage:Accumulates steadily during G2 and is abruptly destroyed at mitosis.,Function:Essential for the control of the cell cycle at the G2/M (mitosis) transition.,PTM:Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin AB subfamily.,subunit:Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.,
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Cellular Localization:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
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Tissue Expression:
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Research Areas:
>>FoxO signaling pathway ;
>>Cell cycle ;
>>Oocyte meiosis ;
>>p53 signaling pathway ;
>>Cellular senescence ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection
>>Cell cycle ;
>>Oocyte meiosis ;
>>p53 signaling pathway ;
>>Cellular senescence ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection
show all
Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0691
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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