Catalog: YP0680
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
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Collected
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Main Information
Target
WAVE1 Phospho Tyr125
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ELISA
MW
70kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ETyDV
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
70kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
Related Products
Primary Antibodies
WAVE1 Rabbit pAb
YT4897
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Primary Antibodies
WAVE1 (Phospho Tyr125) Rabbit pAb
YP0680
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ELISA Kits
Total WAVE1 Cell-Based Colorimetric ELISA Kit
KA4323C
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ELISA Kits
WAVE1 (Phospho Tyr125) Cell-Based Colorimetric ELISA Kit
KA1349C
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human WAVE1 around the phosphorylation site of Tyr125. AA range:91-140
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Specificity:
Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ETyDV
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Gene Name:
WASF1
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Protein Name:
Wiskott-Aldrich syndrome protein family member 1
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Other Name:
WASF1 ;
KIAA0269 ;
SCAR1 ;
WAVE1 ;
Wiskott-Aldrich syndrome protein family member 1 ;
WASP family protein member 1 ;
Protein WAVE-1 ;
Verprolin homology domain-containing protein 1
KIAA0269 ;
SCAR1 ;
WAVE1 ;
Wiskott-Aldrich syndrome protein family member 1 ;
WASP family protein member 1 ;
Protein WAVE-1 ;
Verprolin homology domain-containing protein 1
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Database Link:
Background:
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
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Function:
Domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,Function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.,subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2.,tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.,
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Cellular Localization:
Cytoplasm, cytoskeleton . Cell junction, synapse . Cell junction, focal adhesion . Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity). .
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Tissue Expression:
Research Areas:
>>Adherens junction ;
>>Fc gamma R-mediated phagocytosis ;
>>Regulation of actin cytoskeleton ;
>>Bacterial invasion of epithelial cells ;
>>Pathogenic Escherichia coli infection ;
>>Shigellosis ;
>>Choline metabolism in cancer
>>Fc gamma R-mediated phagocytosis ;
>>Regulation of actin cytoskeleton ;
>>Bacterial invasion of epithelial cells ;
>>Pathogenic Escherichia coli infection ;
>>Shigellosis ;
>>Choline metabolism in cancer
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0680
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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