Catalog: YP1877
Size
Price
Status
Qty.
200μL
$936.00
3 weeks
0
100μL
$560.00
3 weeks
0
50μL
$300.00
3 weeks
0
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Collected
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Main Information
Target
eIF2α Phospho Ser51
Host Species
Mouse
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ELISA
MW
36kD (Calculated)
40kD,90kD (Observed)
Conjugate/Modification
phosphate
Detailed Information
Recommended Dilution Ratio
WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Specificity
This antibody detects endogenous levels of eIF2α (Phospho Ser51) protein.
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage
-15°C to -25°C/1 year (Do not lower than -25°C)
Concentration
1 mg/ml
MW(Calculated)
36kD
MW(Observed)
40kD,90kD
Modification
phosphate
Clonality
Monoclonal
Clone Number
PTR1316
Isotype
IgG2b, Kappa
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Antigen&Target Information
Immunogen:
Synthesized peptide derived from human eIF2α AA range: 1-100
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Specificity:
This antibody detects endogenous levels of eIF2α (Phospho Ser51) protein.
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Gene Name:
EIF2S1 EIF2A
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Protein Name:
Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic translation initiation factor 2 subunit alpha) (eIF-2-alpha) (eIF-2A) (eIF-2alpha)
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Database Link:
Background:
eukaryotic translation initiation factor 2 subunit alpha (EIF2S1) Homo sapiens The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation , promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA , EIF2 , and GTP. EIF2 is composed of 3 nonidentical subunits , the 36-kD EIF2-alpha subunit (EIF2S1) , the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908) , and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. , 1987 [PubMed 2948954]) .[supplied by OMIM , Feb 2010] ,
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Function:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA . This complex binds to a 40S ribosomal subunit , followed by mRNA binding to form a 43S pre-initiation complex . Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex . In order for eIF-2 to recycle and catalyze another round of initiation , the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B . EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR) , required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI , EIF2AK2/PKR , EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor , leading to an attenuation of cap-dependent translation , while concomitantly initiating the preferential translation of ISR-specific mRNAs , such as the transcriptional activators ATF4 and QRICH1 , and hence allowing ATF4- and QRICH1-mediated reprogramming .
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Signaling Pathway
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Cellular Processes >> Cell growth and death >> Apoptosis
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Human Diseases >> Neurodegenerative disease >> Parkinson disease
Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Human Diseases >> Neurodegenerative disease >> Prion disease
Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases
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Catalog: YP1877
Size
Price
Status
Qty.
200μL
$936.00
3 weeks
0
100μL
$560.00
3 weeks
0
50μL
$300.00
3 weeks
0
Add to cart
Collected
Collect
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