Catalog: YP0054
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
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Main Information
Target
CDK1/CDC2 Phospho Tyr15
Host Species
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, ELISA
MW
34kD (Observed)
Conjugate/Modification
phosphate
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Cdc2 (Y15) Polyclonal Antibody detects endogenous levels of Cdc2 protein only when phosphorylated at Y15.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GTyGV
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
34kD
Modification
phosphate
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Tyr15. AA range:5-54
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Specificity:
Phospho-Cdc2 (Y15) Polyclonal Antibody detects endogenous levels of Cdc2 protein only when phosphorylated at Y15.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GTyGV
show all
Gene Name:
CDK1
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Protein Name:
Cyclin-dependent kinase 1
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Other Name:
CDK1 ;
CDC2 ;
CDC28A ;
CDKN1 ;
P34CDC2 ;
Cyclin-dependent kinase 1 ;
CDK1 ;
Cell division control protein 2 homolog ;
Cell division protein kinase 1 ;
p34 protein kinase
CDC2 ;
CDC28A ;
CDKN1 ;
P34CDC2 ;
Cyclin-dependent kinase 1 ;
CDK1 ;
Cell division control protein 2 homolog ;
Cell division protein kinase 1 ;
p34 protein kinase
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Database Link:
Background:
cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
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Function:
Catalytic activity:ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.,Function:Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.,
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Cellular Localization:
Nucleus. Cytoplasm. Mitochondrion . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle. Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
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Tissue Expression:
Research Areas:
>>Cell cycle ;
>>Oocyte meiosis ;
>>p53 signaling pathway ;
>>Cellular senescence ;
>>Gap junction ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection ;
>>Viral carcinogenesis
>>Oocyte meiosis ;
>>p53 signaling pathway ;
>>Cellular senescence ;
>>Gap junction ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection ;
>>Viral carcinogenesis
show all
Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0054
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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