Catalog: YP0051
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
Main Information
Target
CD3 ζ Phospho Tyr142
Host Species
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ELISA
MW
20kD (Observed)
Conjugate/Modification
phosphate
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GLyQG
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
20kD
Modification
phosphate
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range:111-160
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Specificity:
Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GLyQG
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Gene Name:
CD247
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Protein Name:
T-cell surface glycoprotein CD3 zeta chain
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Other Name:
CD247 ;
CD3Z ;
T3Z ;
TCRZ ;
T-cell surface glycoprotein CD3 zeta chain ;
T-cell receptor T3 zeta chain ;
CD antigen CD247
CD3Z ;
T3Z ;
TCRZ ;
T-cell surface glycoprotein CD3 zeta chain ;
T-cell receptor T3 zeta chain ;
CD antigen CD247
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Background:
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
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Function:
Disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,Domain:The ITAM domains mediate interaction with SHB.,Function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts with DOCK2 and TRAT1. Interacts with HIV-1 Nef protein. Interacts with SHB.,
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Cellular Localization:
Cell membrane ; Single-pass type I membrane protein.
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Tissue Expression:
Research Areas:
>>Natural killer cell mediated cytotoxicity ;
>>Th1 and Th2 cell differentiation ;
>>Th17 cell differentiation ;
>>T cell receptor signaling pathway ;
>>Chagas disease ;
>>Epstein-Barr virus infection ;
>>Human immunodeficiency virus 1 infection ;
>>PD-L1 expression and PD-1 checkpoint pathway in cancer
>>Th1 and Th2 cell differentiation ;
>>Th17 cell differentiation ;
>>T cell receptor signaling pathway ;
>>Chagas disease ;
>>Epstein-Barr virus infection ;
>>Human immunodeficiency virus 1 infection ;
>>PD-L1 expression and PD-1 checkpoint pathway in cancer
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Signaling Pathway
Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity
Organismal Systems >> Immune system >> T cell receptor signaling pathway
Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation
Organismal Systems >> Immune system >> Th17 cell differentiation
Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer
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Catalog: YP0051
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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