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DDDDK-Tag(binds to flag sequnence) Rabbit pAb

-YG0004

Main Information
Target

FLAG-tag

Host Species

Rabbit

Reactivity

Species independent

Applications

WB, ELISA, IP, IF

Conjugate/Modification


Unmodified

Detailed Information
Recommended Dilution Ratio
WB 1:1000-1:3000; ELISA 1:5000-20000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
Modification
Unmodified
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
DDDDK synthetic peptide conjugated to KLH.
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Specificity:
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
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Gene Name:
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
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Other Name:
DDDDK epitope tag ;
DDDDK epitope tag ;
DYKDDDDK epitope tag
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Background:
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
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