Catalog: YG0002
Size
Price
Status
Qty.
1mL
$1,360.00
3 weeks
0
500μL
$800.00
3 weeks
0
100μL
$184.00
3 weeks
0
Add to cart
Collected
Collect
Conjugated antibody
His-Tag (4E6) Mouse mAb (AbFluor 350)
His-Tag (4E6) Mouse mAb (AbFluor 405)
His-Tag (4E6) Mouse mAb (AbFluor 488)
His-Tag (4E6) Mouse mAb (AbFluor 555)
His-Tag (4E6) Mouse mAb (AbFluor 594)
His-Tag (4E6) Mouse mAb (AbFluor 647)
His-Tag (4E6) Mouse mAb (AbFluor 680)
His-Tag (4E6) Mouse mAb (Cy3)
His-Tag (4E6) Mouse mAb (Cy5)
His-Tag (4E6) Mouse mAb (FITC)
His-Tag Rabbit pAb (HRP)
His-Tag (4E6) Mouse mAb (HRP)
Main Information
Target
His-tag
Host Species
Rabbit
Reactivity
Species independent
Applications
WB, ELISA
MW
15kD (Observed)
Conjugate/Modification
Unmodified
Detailed Information
Recommended Dilution Ratio
WB 1:1000-1:3000; ELISA 1:20000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
15kD
Modification
Unmodified
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
6X His synthetic peptide conjugated to KLH.
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Specificity:
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
show all
Gene Name:
His-Tag
show all
Protein Name:
His Tag
show all
Other Name:
6 His epitope tag ;
Hexa His tag ;
HHHHHH epitope tag ;
HHHHHH tag ;
His tag
Hexa His tag ;
HHHHHH epitope tag ;
HHHHHH tag ;
His tag
show all
Background:
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
show all
Reference Citation({{totalcount}})
Catalog: YG0002
Size
Price
Status
Qty.
1mL
$1,360.00
3 weeks
0
500μL
$800.00
3 weeks
0
100μL
$184.00
3 weeks
0
Add to cart
Collected
Collect
Conjugated antibody
His-Tag (4E6) Mouse mAb (AbFluor 350)
His-Tag (4E6) Mouse mAb (AbFluor 405)
His-Tag (4E6) Mouse mAb (AbFluor 488)
His-Tag (4E6) Mouse mAb (AbFluor 555)
His-Tag (4E6) Mouse mAb (AbFluor 594)
His-Tag (4E6) Mouse mAb (AbFluor 647)
His-Tag (4E6) Mouse mAb (AbFluor 680)
His-Tag (4E6) Mouse mAb (Cy3)
His-Tag (4E6) Mouse mAb (Cy5)
His-Tag (4E6) Mouse mAb (FITC)
His-Tag Rabbit pAb (HRP)
His-Tag (4E6) Mouse mAb (HRP)
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