Catalog: YM9224
Size
Price
Status
Qty.
200μL
$600.00
3 weeks
0
100μL
$340.00
3 weeks
0
40μL
$190.00
3 weeks
0
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Collected
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Main Information
Target
Cdc25C Phospho Ser216
Host Species
Rabbit
Reactivity
Human, Mouse
Applications
WB, IHC, IF, IP, ELISA
MW
53kD (Calculated)
58kD (Observed)
Conjugate/Modification
phosphate
Detailed Information
Recommended Dilution Ratio
IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Specificity
Cdc25C (Phospho Ser216) Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SPsMP
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year (Do not lower than -25°C)
MW(Calculated)
53kD
MW(Observed)
58kD
Modification
phosphate
Clonality
Monoclonal
Clone Number
PT1382R
Isotype
IgG, Kappa
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Antigen&Target Information
Immunogen:
The specific immunogen used to produce this antibody is proprietary information.
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Specificity:
Cdc25C (Phospho Ser216) Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SPsMP
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Gene Name:
CDC25C
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Protein Name:
M-phase inducer phosphatase 3
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Other Name:
CDC25C ;
M-phase inducer phosphatase 3 ;
Dual specificity phosphatase Cdc25C
M-phase inducer phosphatase 3 ;
Dual specificity phosphatase Cdc25C
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Background:
cell division cycle 25C (CDC25C) Homo sapiens This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq , Dec 2015] ,
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Function:
Catalytic activity:Protein tyrosine phosphate + H (2) O = protein tyrosine + phosphate. ,developmental stage:Expressed predominantly in G2 phase. ,Function:Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity. ,PTM:Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. ,similarity:Belongs to the MPI phosphatase family. ,similarity:Contains 1 rhodanese domain. ,subunit:Interacts with HIV-1 Vpr , thereby inactivating CDC25C phosphatase activity. ,
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Cellular Localization:
Nucleus .
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Tissue Expression:
Research Areas:
>>Cell cycle ;
>>Oocyte meiosis ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection ;
>>MicroRNAs in cancer
>>Oocyte meiosis ;
>>Progesterone-mediated oocyte maturation ;
>>Human immunodeficiency virus 1 infection ;
>>MicroRNAs in cancer
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YM9224
Size
Price
Status
Qty.
200μL
$600.00
3 weeks
0
100μL
$340.00
3 weeks
0
40μL
$190.00
3 weeks
0
Add to cart
Collected
Collect
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