Catalog: YP0331
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
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50μL
$190.00
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Main Information
Target
Pim-1 Phospho Tyr309
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
MW
45kD (Observed)
Conjugate/Modification
Phospho
Detailed Information
Recommended Dilution Ratio
WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):HRyHG
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
1 mg/ml
MW(Observed)
45kD
Modification
Phospho
Clonality
Polyclonal
Isotype
IgG
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Antigen&Target Information
Immunogen:
The antiserum was produced against synthesized peptide derived from human Pim-1 around the phosphorylation site of Tyr309. AA range:281-330
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Specificity:
Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):HRyHG
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Gene Name:
PIM1
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Protein Name:
Serine/threonine-protein kinase pim-1
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Other Name:
PIM1 ;
Serine/threonine-protein kinase pim-1
Serine/threonine-protein kinase pim-1
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Background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011],
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Function:
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Manganese.,Function:Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.,induction:Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines.,PTM:Autophosphorylated on both serine/threonine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.,tissue specificity:Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.,
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Cellular Localization:
[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cell membrane.
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Tissue Expression:
Research Areas:
>>JAK-STAT signaling pathway ;
>>AGE-RAGE signaling pathway in diabetic complications ;
>>Pathways in cancer ;
>>MicroRNAs in cancer ;
>>Acute myeloid leukemia
>>AGE-RAGE signaling pathway in diabetic complications ;
>>Pathways in cancer ;
>>MicroRNAs in cancer ;
>>Acute myeloid leukemia
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Signaling Pathway
Reference Citation({{totalcount}})
Catalog: YP0331
Size
Price
Status
Qty.
200μL
$600.00
In stock
0
100μL
$340.00
In stock
0
50μL
$190.00
In stock
0
Add to cart
Collected
Collect
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