Integrin α4 Polyclonal Antibody

    • Catalog No.:YT5248
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
    • Description
    • Gene Name:
    • ITGA4
    • Protein Name:
    • Integrin alpha-4
    • Human Gene Id:
    • 3676
    • Human Swiss Prot No:
    • P13612
    • Mouse Swiss Prot No:
    • Q00651
    • Immunogen:
    • The antiserum was produced against synthesized peptide derived from the Internal region of human ITGA4. AA range:571-620
    • Specificity:
    • Integrin α4 Polyclonal Antibody detects endogenous levels of Integrin α4 protein.
    • Formulation:
    • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    • Source:
    • Rabbit
    • Dilution:
    • Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
    • Purification:
    • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    • Concentration:
    • 1 mg/ml
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • ITGA4; CD49D; Integrin alpha-4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD49d
    • MolecularWeight(Da):
    • 115333
    • Observed Band(KD):
    • 120
    • Background:
    • integrin subunit alpha 4(ITGA4) Homo sapiens The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015],
    • Function:
    • domain:The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.,function:Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.,PTM:Phosphorylation on Ser-1027 inhibits PXN binding.,similarity:Belongs to the integrin alpha chain family.,similarity:Contains 7 FG-GAP repeats.,subunit:Heterodimer of an alpha and a beta subunit
    • Subcellular Location:
    • plasma membrane,focal adhesion,integrin complex,cell surface,membrane,integral component of membrane,integrin alpha4-beta7 complex,extracellular exosome,
    • Expression:
    • Lymph,Peripheral blood,Prostate,Spleen,Testis,
    • Products Images
    • Western Blot analysis of K562 cells using Integrin α4 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
    • Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
    • Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
    • Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
    • Immunohistochemical analysis of paraffin-embedded mouse-spleen, antibody was diluted at 1:100
    • Immunohistochemical analysis of paraffin-embedded mouse-spleen, antibody was diluted at 1:100