• Target：
• IL-10

• Fields：
• >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>FoxO signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>T cell receptor signaling pathway;>>Intestinal immune network for IgA production;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>African trypanosomiasis;>>Malaria;>>Toxoplasmosis;>>Amoebiasis;>>Staphylococcus aureus infection;>>Tuberculosis;>>Asthma;>>Autoimmune thyroid disease;>>Inflammatory bowel disease;>>Systemic lupus erythematosus;>>Allograft rejection

• Gene Name：
• IL10

• Protein Name：
• Interleukin-10

• Human Gene Id：
• 3586

• Human Swiss Prot No：
• P22301

• Mouse Swiss Prot No：
• P18893

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120

• Specificity：
• IL-10 Polyclonal Antibody detects endogenous levels of IL-10 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IL10;Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF

• Observed Band(KD)：
• 20kD

• Background：
• The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011],

• Function：
• disease:Defects in IL10 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease.,function:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.,online information:Interleukin-10 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-10 family.,subunit:Homodimer.,tissue specificity:Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.,

• Subcellular Location：
• Secreted.

• Expression：
• Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Astragaloside IV alleviates cytarabine-induced intestinal mucositis by remodeling macrophage polarization through AKT signaling PHYTOMEDICINE Jun-jie LI, Ya-ling LI, Wei CHU, Gao-qin LI, Min ZHANG, Juan-juan DONG, Ling LI, Cheng-hao LI, Jin-bao ZHANG, Jia-wei LI, Xiao-jie JIN, Yong-qi LIU WB Mouse intestinal RAW264.7 cell

IL-10/STAT3 is reduced in childhood obesity with hypertriglyceridemia and is related to triglyceride level in diet-induced obese rats. BMC Endocrine Disorders Bmc Endocr Disord. 2018 Dec;18(1):1-9 IHC Rat perirenal adipose tissue,epididymal adipose tissue

Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells. Biomed Research International Biomed Res Int. 2017;2017:3519745 WB Mouse 1 : 1000 imDCs

Hui, Zhi, and Zhe Li. "Continuous veno-venous hemofiltration for septic shock." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10.10 (2017): 14983-14988.

Guiqi Baizhu prescription ameliorates cytarabine-induced intestinal mucositis by targeting JAK2 to inhibit M1 macrophage polarization. Yong-qi Liu WB Mouse 1:1000 ileal tissue M1 macrophage (M1)

Mannosylated polydopamine nanoparticles alleviate radiation- induced pulmonary fibrosis by targeting M2 macrophages and inhibiting the TGF-β1/Smad3 signaling pathway. Yuhui Hao WB Mouse lung tissue

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage. Qi Chen WB Rat,Mouse skin tissue RAW264.7 cell

IL-10-TG/TPO-T4 axis, the target of bis (2-ethylhexyl) tetrabromophthalate on thyroid function imbalance TOXICOLOGY Yuese Yuan WB Rat,Human thyroid tissue Nthy-ori 3–1 cell

Neuroprotective Effect of a Multistrain Probiotic Mixture in SOD1G93A Mice by Reducing SOD1 Aggregation and Targeting the Microbiota-Gut-Brain Axis MOLECULAR NEUROBIOLOGY Xin Zikai WB Mouse 1:500 colon tissue

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