TCF-4/12 Polyclonal Antibody

    • Catalog No.：YT4580
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse;Rat
      • Target：
      • TCF-4/12
      • Gene Name：
      • TCF4/TCF12
      • Protein Name：
      • Transcription factor 4/12
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human TCF4/12. AA range:581-630
      • Specificity：
      • TCF-4/12 Polyclonal Antibody detects endogenous levels of TCF-4/12 protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • TCF4;BHLHB19;ITF2;SEF2;Transcription factor 4;TCF-4;Class B basic helix-loop-helix protein 19;bHLHb19;Immunoglobulin transcription factor 2;ITF-2;SL3-3 enhancer factor 2;SEF-2;TCF12;BHLHB20;HEB;HTF4;Transcription factor 12;
      • Observed Band(KD)：
      • 60kD
      • Background：
      • This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016],
      • Function：
      • disease:Defects in TCF4 are a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954].,disease:Haploinsufficiency of TCF4 is a cause of Pitt-Hopkins syndrome (PTHS) [MIM:610954]. PTHS is a rare syndromic encephalopathy characterized by severe psychomotor delay, epilepsy, daily bouts of diurnal hyperventilation starting in infancy, mild postnatal growth retardation, postnatal microcephaly, and distinctive facial features. Since most hitherto reported cases have been sporadic, with males and females equally affected, PTHS is regarded as an autosomal dominant condition.,function:Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.,sequence caution:Incomplete and probable erro
      • Subcellular Location：
      • Nucleus .
      • Expression：
      • Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
      • Products Images
      • Western Blot analysis of various cells using TCF-4/12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western blot analysis of lysates from HUVEC cells, using TCF4/12 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

      Attributes

      • Code：
      • YT4580
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 150 / 280 / 450
      • Delivery：
      • 2024-05-19
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