• Target：
• Rock-2

• Fields：
• >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>Sphingolipid signaling pathway;>>Vascular smooth muscle contraction;>>Wnt signaling pathway;>>Axon guidance;>>Focal adhesion;>>Tight junction;>>Platelet activation;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Oxytocin signaling pathway;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Lipid and atherosclerosis

• Gene Name：
• ROCK2

• Protein Name：
• Rho-associated protein kinase 2

• Human Gene Id：
• 9475

• Human Swiss Prot No：
• O75116

• Mouse Gene Id：
• 19878

• Mouse Swiss Prot No：
• P70336

• Rat Gene Id：
• 25537

• Rat Swiss Prot No：
• Q62868

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ROCK2. AA range:691-740

• Specificity：
• Rock-2 Polyclonal Antibody detects endogenous levels of Rock-2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunocytochemistry: 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ROCK2;KIAA0619;Rho-associated protein kinase 2;Rho kinase 2;Rho-associated; coiled-coil-containing protein kinase 2;Rho-associated, coiled-coil-containing protein kinase II;ROCK-II;p164 ROCK-2

• Observed Band(KD)：
• 165kD

• Background：
• The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by RHOA binding.,function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.,miscellaneous:Inhibited by Y-27632.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Cytoplasmic, and associated with actin microfilaments and the plasma membrane.,subunit:Homodim

• Subcellular Location：
• Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .

• Expression：
• Expressed in the brain (at protein level).

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