• Target：
• PFK-2 liv/tes

• Fields：
• >>Fructose and mannose metabolism;>>Metabolic pathways;>>AMPK signaling pathway;>>Glucagon signaling pathway

• Gene Name：
• PFKFB1/PFKFB4

• Protein Name：
• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1/4

• Human Gene Id：
• 5207/5210

• Human Swiss Prot No：
• P16118/Q16877

• Mouse Gene Id：
• 18639/270198

• Rat Gene Id：
• 24638/54283

• Rat Swiss Prot No：
• P07953/P25114

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PFKFB1/4. AA range:331-380

• Specificity：
• PFK-2 liv/tes Polyclonal Antibody detects endogenous levels of PFK-2 liv/tes protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PFKFB1;F6PK;PFRX;6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 1;6PF-2-K/Fru-2,6-P2ase 1;PFK/FBPase 1;6PF-2-K/Fru-2,6-P2ase liver isozyme;PFKFB4;6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4;6PF-2-K/Fru-2,6-P2ase 4;

• Observed Band(KD)：
• 54kD

• Background：
• This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012],

• Function：
• catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in inhibition of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Liver.,

• Subcellular Location：
• cytosol,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex,

• Expression：
• Liver.

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