• Target：
• NOS2/iNOS

• Fields：
• >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>Peroxisome;>>Apelin signaling pathway;>>Relaxin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Pertussis;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Amoebiasis;>>Tuberculosis;>>Pathways in cancer;>>Small cell lung cancer

• Gene Name：
• NOS2, INOS

• Protein Name：
• Nitric oxide synthase inducible

• Human Gene Id：
• 4843

• Human Swiss Prot No：
• P35228

• Mouse Gene Id：
• 18126

• Mouse Swiss Prot No：
• P29477

• Rat Gene Id：
• 24599

• Rat Swiss Prot No：
• Q06518

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166

• Specificity：
• NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NOS2;NOS2A;Nitric oxide synthase; inducible;Hepatocyte NOS;HEP-NOS;Inducible NO synthase;Inducible NOS;iNOS;NOS type II;Peptidyl-cysteine S-nitrosylase NOS2

• Observed Band(KD)：
• 131kD

• Background：
• Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Bin

• Subcellular Location：
• Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .

• Expression：
• Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305).

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Zhu, Xiao-Ming, et al. "PM2. 5 induces autophagy-mediated cell death via NOS2 signaling in human bronchial epithelium cells." International journal of biological sciences 14.5 (2018): 557.

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Euphohelides A–C, ent-Abietane-Type Norditerpene Lactones from Euphorbia helioscopia and Their Anti-Inflammatory Activities JOURNAL OF NATURAL PRODUCTS Kun Gao WB Mouse RAW264.7 cell

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