• Target：
• IGF-IR

• Fields：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>Endocytosis;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Focal adhesion;>>Adherens junction;>>Signaling pathways regulating pluripotency of stem cells;>>Long-term depression;>>Ovarian steroidogenesis;>>Progesterone-mediated oocyte maturation;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Proteoglycans in cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Breast cancer;>>Hepatocellular carcinoma

• Gene Name：
• IGF1R

• Protein Name：
• Insulin-like growth factor 1 receptor

• Human Gene Id：
• 3480/3643

• Human Swiss Prot No：
• P08069/P06213

• Mouse Gene Id：
• 16001/16337

• Rat Gene Id：
• 25718

• Rat Swiss Prot No：
• P24062/P15127

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human IGF1R. AA range:1126-1175

• Specificity：
• IGF-IR Polyclonal Antibody detects endogenous levels of IGF-IR protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• IGF1R;Insulin-like growth factor 1 receptor;Insulin-like growth factor I receptor;IGF-I receptor;CD antigen CD221;INSR;Insulin receptor;IR;CD antigen CD220

• Observed Band(KD)：
• pro: 155kD, recetor beta: 95kD

• Background：
• This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],

• Function：
• catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.,enzyme regulation:Autophosphorylation activates the kinase activity.,function:This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low a

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein .

• Expression：
• Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.

Amelioration of Diabetic Mouse Nephropathy by Catalpol Correlates with Down-Regulation of Grb10 Expression and Activation of Insulin-Like Growth Factor 1 / Insulin-Like Growth Factor 1 Receptor Signaling. PLoS One Plos One. 2016 Mar;11(3):e0151857 WB Mouse 1:1000 kidney cortex tissues

Negative Regulation of Grb10 Interacting GYF Protein 2 on Insulin-Like Growth Factor-1 Receptor Signaling Pathway Caused Diabetic Mice Cognitive Impairment. PLoS One 2014 Sep 30 WB Mouse 1∶1000 hippocampus

Insulin-like growth factor II mRNA binding protein 3 regulates proliferation, invasion and migration of neuroendocrine cancer cells. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2017; 10(10): 10269–10275 WB Mouse STC-1 cell

Expression of IMP3 as a marker for predicting poor outcome in gastroenteropancreatic neuroendocrine neoplasms. Oncology Letters 2017 Feb 14 IHC Human 1:400 gastroenteropancreatic neuroendocrine neoplasm (GEP‑NEN)

Marine bromophenol bis (2, 3-dibromo-4, 5-dihydroxybenzyl) ether, represses angiogenesis in HUVEC cells and in zebrafish embryos via inhibiting the VEGF signal systems." Biomedicine & Pharmacotherapy 75 (2015): 58-66.

Amelioration of Diabetic Mouse Nephropathy by Catalpol Correlates with Down-Regulation of Grb10 Expression and Activation of Insulin-Like Growth Factor 1 / Insulin-Like Growth Factor 1 Receptor Signaling. PLoS One Plos One. 2016 Mar;11(3):e0151857 WB Mouse 1:1000 kidney cortex tissues

Amelioration of Diabetic Mouse Nephropathy by Catalpol Correlates with Down-Regulation of Grb10 Expression and Activation of Insulin-Like Growth Factor 1 / Insulin-Like Growth Factor 1 Receptor Signaling. PLoS One Plos One. 2016 Mar;11(3):e0151857 WB Mouse 1:1000 kidney cortex tissues

Clinico-pathological characteristics of IGFR1 and VEGF-A co-expression in early and locally advanced-stage lung adenocarcinoma. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY Qin Tingting IHC Human 1:800 lung adenocarcinoma tissue

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