Frizzled-7 Polyclonal Antibody

    • Catalog No.：YT1786
    • Applications：WB;FCM;IF;ELISA
    • Reactivity：Human;Mouse
      • Target：
      • Frizzled-7
      • Fields：
      • >>mTOR signaling pathway;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
      • Gene Name：
      • FZD7
      • Protein Name：
      • Frizzled-7
      • Human Gene Id：
      • 8324
      • Human Swiss Prot No：
      • O75084
      • Mouse Swiss Prot No：
      • Q61090
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human FZD7. AA range:45-94
      • Specificity：
      • Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500-2000;Flow Cyt 1:50-200;IF ICC 1:100-500;ELISA 1:5000-20000
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • FZD7;Frizzled-7;Fz-7;hFz7;FzE3
      • Observed Band(KD)：
      • 64kD
      • Background：
      • frizzled class receptor 7(FZD7) Homo sapiens Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008],
      • Function：
      • domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog
      • Subcellular Location：
      • Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2). Localized in recycling endosomes in other conditions. .
      • Expression：
      • High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.
      • Products Images
      • Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
      • Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
      • Western Blot analysis of HELA cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000
      • Immunofluorescence analysis of MCF7 cells, using FZD7 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HT-29 cells, using FZD7 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from HUVECcells using FZD7 antibody.

      Attributes

      • Code：
      • YT1786
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 150 / 280 / 450
      • Delivery：
      • 2024-05-19
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