CREB-1 Polyclonal Antibody

    • Catalog No.:YT1097
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • CREB-1
      • Fields:
      • >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Adrenergic signaling in cardiomyocytes;>>Osteoclast differentiation;>>Antigen processing and presentation;>>TNF signaling pathway;>>Circadian rhythm;>>Circadian entrainment;>>Thermogenesis;>>Cholinergic synapse;>>Dopaminergic synapse;>>Insulin secretion;>>Estrogen signaling pathway;>>Melanogenesis;>>Thyroid hormone synthesis;>>Glucagon signaling pathway;>>Renin secretion;>>Aldosterone synthesis and secretion;>>Relaxin signaling pathway;>>Cortisol synthesis and secretion;>>Parathyroid hormone synthesis, secretion and action;>>Insulin resistance;>>Cushing syndrome;>>Growth hormone synthesis, secretion and action;>>Vasopressin-regulated water reabsorption;>>Huntington disease;>>Prion disease;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism;>>Tuberculosis;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human
      • Gene Name:
      • CREB1
      • Protein Name:
      • Cyclic AMP-responsive element-binding protein 1
      • Human Gene Id:
      • 1385
      • Human Swiss Prot No:
      • P16220
      • Mouse Swiss Prot No:
      • Q01147
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CREB. AA range:100-149
      • Specificity:
      • CREB-1 Polyclonal Antibody detects endogenous levels of CREB-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CREB1;Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1
      • Observed Band(KD):
      • 37kD
      • Background:
      • This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
      • Function:
      • disease:A chromosomal aberration involving CREB1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.,function:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.,PTM:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.,similarit
      • Subcellular Location:
      • Nucleus .
      • Expression:
      • Eye,Placenta,Spleen,Testis,

      Pituitary miRNAs target GHRHR splice variants to regulate GH synthesis by mediating different intracellular signalling pathways. RNA Biology Rna Biol. 2020;17(12):1754-1766 WB Pig,Rat primary pituitary cell,GH3 cell

      miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2020 Apr;506:110763 WB Rat GH3 cell

      Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood. Brain and Behavior Brain Behav. 2020 Oct;10(10):e01801 WB Rat 1 : 1000 Hippocampal tissue

      Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2018 Aug;19(8):2241 WB Rat 1:1000 hippocampal tissue

      Cyclooxygenase-2 up-regulates hepatic somatostatin receptor 2 expression. Scientific Reports Sci Rep-Uk. 2018 Jul;8(1):1-11 WB Rat,Human cirrhotic liver L02 cell

      Dichloroacetic acid-induced dysfunction in rat hippocampus and the protective effect of curcumin. METABOLIC BRAIN DISEASE Metab Brain Dis. 2021 Apr;36(4):545-556 WB Rat 1:1200 Hippocampus

      Lu, Qian, et al. "Norisoboldine suppresses VEGF-induced endothelial cell migration via the cAMP-PKA-NF-κB/Notch1 pathway." PloS one 8.12 (2013): e81220.

      Li, Bai, et al. "Developmental exposure to paraquat and maneb can impair cognition, learning and memory in Sprague-Dawley rats." Molecular BioSystems 12.10 (2016): 3088-3097.

      • Products Images
      • Wang, Yue, et al. "Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood." Brain and Behavior 10.10 (2020): e01801.
      • Lu, Qian, et al. "Norisoboldine suppresses VEGF-induced endothelial cell migration via the cAMP-PKA-NF-κB/Notch1 pathway." PloS one 8.12 (2013): e81220.
      • Western Blot analysis of various cells using CREB-1 Polyclonal Antibody diluted at 1:1000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CREB Antibody. The picture on the right is blocked with the synthesized peptide.