Actin Polyclonal Antibody
- Catalog No.:YT0096
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Fish
- Target:
- Actin pan
- Fields:
- >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis
- Gene Name:
- ACTG1
- Protein Name:
- Actin cytoplasmic 2
- Human Gene Id:
- 60/71
- Human Swiss Prot No:
- P60709/Q9BYX7/P63261
- Mouse Gene Id:
- 11461/11465
- Rat Gene Id:
- 81822/100361457
- Rat Swiss Prot No:
- P60711/P63259
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Actin. AA range:326-375
- Specificity:
- Actin Polyclonal Antibody detects endogenous levels of Actin protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- ACTB;Actin; cytoplasmic 1;Beta-actin;POTEKP;ACTBL3;FKSG30;Putative beta-actin-like protein 3;Kappa-actin;POTE ankyrin domain family member K;ACTG1;ACTB;ACTG;Actin, cytoplasmic 2;Gamma-actin
- Observed Band(KD):
- 45kD
- Background:
- This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
- Subcellular Location:
- Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
- Expression:
- B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
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Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein–Barr virus entry into epithelial cells Nature Microbiology Yang Yinggui WB Human 1:5000 NP69 cell,HK1 cell,HEK293 cell
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Actin Polyclonal Antibody diluted at 1:2000
- Western Blot analysis of HeLa cells using Actin Polyclonal Antibody diluted at 1:2000
- Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of various lysis using Actin Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actin-pan Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa cells, using Actin-pan Antibody. The lane on the right is blocked with the synthesized peptide.