IRS-1 (phospho Ser312) Polyclonal Antibody

    • Catalog No.:YP0571
    • Applications:WB,IF/ICC,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • IRS1
      • Protein Name:
      • Insulin receptor substrate 1
      • Human Gene Id:
      • 3667
      • Human Swiss Prot No:
      • P35568
      • Mouse Swiss Prot No:
      • P35569
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser312. AA range:279-328
      • Specificity:
      • Phospho-IRS-1 (S312) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S312.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • IRS1; Insulin receptor substrate 1; IRS-1
      • MolecularWeight(Da):
      • 131591
      • Observed Band(KD):
      • 170
      • Background:
      • insulin receptor substrate 1(IRS1) Homo sapiens This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],
      • Function:
      • disease:Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].,function:May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.,polymorphism:The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependen
      • Subcellular Location:
      • nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle,
      • Expression:
      • Epithelium,Eye,Skeletal muscle,
      • Products Images
      • Western Blot analysis of various cells using Phospho-IRS-1 (S312) Polyclonal Antibody diluted at 1:1000
      • Western blot analysis of 293T Hela mouse-spleen lysis using Phospho-IRS-1 (S312) antibody. Antibody was diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser312) Antibody
      • Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser312) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HepG2 cells, using IRS-1 (Phospho-Ser312) Antibody. The lane on the right is blocked with the phospho peptide.