• Target：
• p38

• Fields：
• >>Endocrine resistance;>>MAPK signaling pathway;>>Rap1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Neurotrophin signaling pathway;>>Retrograde endocannabinoid signaling;>>Dopaminergic synapse;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Progesterone-mediated oocyte maturation;>

• Gene Name：
• MAPK14

• Protein Name：
• Mitogen-activated protein kinase 14

• Human Gene Id：
• 1432

• Human Swiss Prot No：
• Q16539

• Mouse Gene Id：
• 26416

• Mouse Swiss Prot No：
• P47811

• Rat Swiss Prot No：
• P70618

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200

• Specificity：
• Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF ICC 1:100-500;WB 1:500-2000;Flow Cyt 1:50-200;IHC 1:100-500;ELISA 1:5000-20000

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK14;CSBP;CSBP1;CSBP2;CSPB1;MXI2;SAPK2A;Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein

• Observed Band(KD)：
• 38kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K3 or MAP2K6, and potentially also MAP2K4. Inhibited by dual specificity phosphatases, such as DUSP1. Specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine-suppressive anti-inflammatory drugs (CSAID). Isoform Mxi2 is 100-fold less sensitive to these agents than the other isoforms and is not inhibited by DUSP1. Isoform Exip is not activated by MAP2K6.,function:Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and seve

• Subcellular Location：
• Cytoplasm . Nucleus .

• Expression：
• Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro MICROBIAL PATHOGENESIS Fengyi Zhou, Xin Li, Xiaochi Chang, Zhihao Geng, Wenjing Hao, Jing Deng, Hai Ming Wong, Shuai Wang WB Mouse BMMs

GCN5 participates in KLF4-VEGFA feedback to promote endometrial angiogenesis WB Human /HEMECs

Polydatin Improves Sepsis-Associated Encephalopathy by Activating Sirt1 and Reducing p38 Phosphorylation. JOURNAL OF SURGICAL RESEARCH2022 Aug;276:379. Mouse brain tissue

Therapeutic Potential of Synthetic Human β-Defensin 1 Short Motif Pep-B on Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells Mediat Inflamm. 2022;2022:6141967. WB Human 1 : 1000

Suhuang Antitussive Capsule Ameliorates Corticosteroid Insensitivity in Cough Variant Asthma Guinea Pigs by Inhibiting p38 MAPK Signal Pathway Evid-Based Compl Alt. 2022;2022:1699429. WB Guinea pig 1 : 1000 lung

Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation Food Sci Tech-Brazil. 2022 Mar;42:. WB Mouse 1:500

Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer. Cell Death & Disease Cell Death Dis. 2021 Jun;12(7):1-11 WB Human MDA–MB-231 cells,MDA–MB-468 cells

Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells. Cancer Cell International Cancer Cell Int. 2021 Dec;21(1):1-12 WB Human 1:1000 H460 cell

Astragalus Polysaccharide Inhibits Ionizing Radiation-Induced Bystander Effects by Regulating MAPK/NF-κB Signaling Pathway in Bone Mesenchymal Stem Cells (BMSCs). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 4649–4658 WB Human BMSCs

NOV inhibits proliferation while promoting apoptosis and migration in osteosarcoma cell lines through p38/MAPK and JNK/MAPK pathways. ONCOLOGY REPORTS 2015 Jul 24 WB Human 143B cell

P38 MAPK/AKT signalling is involved in IL-33-mediated anti-apoptosis in childhood acute lymphoblastic leukaemia blast cells. ANNALS OF MEDICINE Ann Med. 2021;53(1):1464-1472 WB Human Bone marrow (BM) cell

Anemonin Attenuates RANKL-Induced Osteoclastogenesis and Ameliorates LPS-Induced Inflammatory Bone Loss in Mice via Modulation of NFATc1. Frontiers in Pharmacology 2020 Feb 10 WB Mouse BMMs

Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. NEUROTOXICOLOGY Neurotoxicology. 2022 Jan;88:187 WB Human 1:500 SH-SY5Y cell

Effect of cold stress on the MAPK pathway and lipidomics on muscle of Takifugu fasciatus. AQUACULTURE Aquaculture. 2021 Jul;540:736691 WB Fish 1:1500 Muscles

Active Peptide KF-8 from Rice Bran Attenuates Oxidative Stress in a Mouse Model of Aging Induced by d-Galactose. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2020;68(44):12271–12283 WB Mouse 1 : 1000 Liver NIH/3T3 cell

The Protective Effects of Lactobacillus plantarum KLDS 1.0344 on LPS-Induced Mastitis In Vitro and In Vivo. Frontiers in Immunology Front Immunol. 2021; 12: 770822 WB Mouse 1:500 Breast

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 WB Mouse RAW264.7 cell

Amelioration of Neuropathic Pain and Attenuation of Neuroinflammation Responses by Tetrahydropalmatine Through the p38MAPK/NF-κB/iNOS Signaling Pathways in Animal and Cellular Models. INFLAMMATION Inflammation. 2021 Nov;:1-13 WB，IF Mouse 1:500，1:200 Dorsal root ganglion (DRG)，sciatic nerve (SN) N2a cells ，BV2 microglia cell

The Apolipoprotein C1 is involved in breast cancer progression via EMT and MAPK/JNK pathway. PATHOLOGY RESEARCH AND PRACTICE Pathol Res Pract. 2022 Jan;229:153746 WB Human Breast cancer tissue MDA-MB-231 cell , MCF-7 cell

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

Autoimmune regulator‑overexpressing dendritic cells induce T helper 1 and T helper 17 cells by upregulating cytokine expression. Molecular Medicine Reports 2016 Jan 01 FC Mouse 1:100 DC2.4 cell,T Lymphocyte

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:500 Acute aortic aortic valve interstitial cells (AVICs)

Pretreatment with pPolyHb attenuates H2O2-induced endothelial cell injury through inhibition of JNK/p38 MAPK pathway by upregulation of heme oxygenase-1. Artificial Cells Nanomedicine and Biotechnology Artif Cell Nanomed B. 2015;43(3):163-173 WB Human 1:500 HUVECs

Li, Pingli, et al. "Preparation and in vitro immunomodulatory effect of curdlan sulfate." Carbohydrate polymers 102 (2014): 852-861.

Wu, Yunhua, et al. "TMIGD1 is Expressed at Low Levels in Abdominal Adhesion Tissue and Reduces Oxidative Stress in Peritoneal Mesothelial Cells." (2020).

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection Cells Jianping Pan WB Human,Mouse

Onychiol B attenuates lipopolysaccharide-induced inflammation via MAPK/NF-κB pathways and acute lung injury in vivo BIOORGANIC CHEMISTRY Guozheng Huang WB Mouse RAW264.7 cells

ZFP36L1 Promotes Gastric Cancer Progression via Regulating JNK and p38 MAPK Signaling Pathways Recent Patents on Anti-Cancer Drug Discovery Fenghua Zhou WB Human SGC-7901, BGC-823 cell

The regulatory role and mechanism of lncTUG1 on cartilage apoptosis and inflammation in osteoarthritis. Xiao-Yu Chen WB Human 1:1000 C28/I2 cell

Muscone attenuates susceptibility to ventricular arrhythmia by inhibiting NLRP3 inflammasome activation in rats after myocardial infarction. Yanhong Tang WB Rat 1:1000 hearts

Anti-inflammatory activities of several diterpenoids isolated from Hemionitis albofusca. Cao Jianguo WB Mouse 1:1000 RAW264.7 cell

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:2000 IEC-6 cell

Microplastics induced inflammation in the spleen of developmental Japanese quail (Coturnix japonica) via ROS-mediated p38 MAPK and TNF signaling pathway activation1 ENVIRONMENTAL POLLUTION Qingyu Zhang WB Bird 1:2000 spleen tissue

Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

Effect of the Pulsatilla decoction n-butanol extract on vulvovaginal candidiasis caused by Candida glabrata and on its virulence factors FITOTERAPIA Jiaping Zhang WB Mouse vaginal tissue

Design, synthesis, in vitro and in vivo biological evaluation of pterostilbene derivatives for anti-inflammation therapy JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

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