CaMKIIα/δ (phospho Thr286) Polyclonal Antibody
- Catalog No.:YP0042
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Pig
- Target:
- CaMK2
- Fields:
- >>ErbB signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>HIF-1 signaling pathway;>>Oocyte meiosis;>>Necroptosis;>>Adrenergic signaling in cardiomyocytes;>>Wnt signaling pathway;>>Axon guidance;>>Circadian entrainment;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Cholinergic synapse;>>Dopaminergic synapse;>>Olfactory transduction;>>Inflammatory mediator regulation of TRP channels;>>Insulin secretion;>>GnRH signaling pathway;>>Melanogenesis;>>Oxytocin signaling pathway;>>Glucagon signaling pathway;>>Aldosterone synthesis and secretion;>>Cushing syndrome;>>Gastric acid secretion;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases;>>Amphetamine addiction;>>Tuberculosis;>>Pathways in cancer;>>Proteoglycans in cancer;>>Glioma;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis
- Gene Name:
- CAMK2A/CAMK2D
- Protein Name:
- Calcium/calmodulin-dependent protein kinase type II subunit alpha/delta
- Human Gene Id:
- 815/817
- Human Swiss Prot No:
- Q9UQM7/Q13557
- Mouse Gene Id:
- 12322/108058
- Rat Gene Id:
- 25400/24246
- Rat Swiss Prot No:
- P11275/P15791
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305
- Specificity:
- Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CAMK2A;CAMKA;KIAA0968;Calcium/calmodulin-dependent protein kinase type II subunit alpha;CaM kinase II subunit alpha;CaMK-II subunit alpha;CAMK2D;CAMKD;Calcium/calmodulin-dependent protein kinase type II subunit delta;CaM kinase II
- Observed Band(KD):
- 54kD
- Background:
- The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.,function:CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Postsynaptic lipid rafts.,subunit:CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric
- Subcellular Location:
- Cell junction, synapse . Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, dendrite . Postsynaptic lipid rafts. .
- Expression:
- Brain,
Mechanism of deoxynivalenol-induced neurotoxicity in weaned piglets is linked to lipid peroxidation, dampened neurotransmitter levels, and interference with calcium signaling. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2020 May;194:110382 WB Pig Brain
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- Antibody-FAQs
- Products Images
- Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 (Phospho-Thr286) Antibody
- Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from K562 cells, using CaMK2 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.