β I tubulin Monoclonal Antibody(3F7)

    • Catalog No.:YM3037
    • Applications:WB,IHC-p,IF,
    • Reactivity:Human,Rat,Mouse
    • Description
    • Gene Name:
    • TUBB1
    • Protein Name:
    • Tubulin beta-1 chain
    • Human Swiss Prot No:
    • Q9H4B7
    • Mouse Swiss Prot No:
    • A2AQ07
    • Immunogen:
    • Synthetic Peptide of β I tubulin
    • Specificity:
    • The antibody detects endgenousβ I tubulin proteins.
    • Formulation:
    • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    • Source:
    • Mouse
    • Dilution:
    • WB: 1:500-10000 IF 1:200 IHC 1:50-300
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • TUBB1; Tubulin beta-1 chain; TUBB2A; TUBB2; Tubulin beta-2A chain; TUBB2B; Tubulin beta-2B chain; TUBB4B; TUBB2C; Tubulin beta-4B chain; Tubulin beta-2 chain; Tubulin beta-2C chain; TUBB3; TUBB4; Tubu
    • Observed Band(KD):
    • 50
    • Background:
    • TUBB1 encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. tubulin b
    • Function:
    • microtubule cytoskeleton organization, M phase, protein complex assembly, cytoskeleton organization,
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunofluorescence analysis of Human-breast-cancer tissue. 1,β I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue, diluted at 1:5000.