β I tubulin Monoclonal Antibody(3F7)

    • Catalog No.:YM3037
    • Applications:WB,IHC-p,IF,
    • Reactivity:Human,Rat,Mouse
    • Description
    • Gene Name:
    • TUBB1
    • Protein Name:
    • Tubulin beta-1 chain
    • Human Swiss Prot No:
    • Q9H4B7
    • Mouse Swiss Prot No:
    • A2AQ07
    • Immunogen:
    • Synthetic Peptide of β I tubulin
    • Specificity:
    • The antibody detects endgenousβ I tubulin proteins.
    • Formulation:
    • PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
    • Source:
    • Mouse
    • Dilution:
    • WB: 1:500-10000 IF 1:200 IHC 1:50-300
    • Purification:
    • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
    • Storage Stability:
    • -20°C/1 year
    • Other Name:
    • TUBB1; Tubulin beta-1 chain; TUBB2A; TUBB2; Tubulin beta-2A chain; TUBB2B; Tubulin beta-2B chain; TUBB4B; TUBB2C; Tubulin beta-4B chain; Tubulin beta-2 chain; Tubulin beta-2C chain; TUBB3; TUBB4; Tubu
    • Observed Band(KD):
    • 50
    • Background:
    • TUBB1 encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. tubulin beta 1 class VI is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in TUBB1 is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of tTUBB1 are found on chromosome Y.
    • Function:
    • microtubule cytoskeleton organization, M phase, protein complex assembly, cytoskeleton organization, microtubule-based process, microtubule-based movement, cell cycle, spindle organization, cell cycle process, cell cycle phase,cellular macromolecular complex subunit organization, cellular macromolecular complex assembly, cellular protein complex assembly, macromolecular complex subunit organization, spindle assembly, protein polymerization,macromolecular complex assembly, protein complex biogenesis,
    • Products Images
    • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,β I tubulin Monoclonal Antibody(3F7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
    • Immunofluorescence analysis of Human-breast-cancer tissue. 1,β I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
    • Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue, diluted at 1:5000.