• Target：
• TIF1β

• Gene Name：
• trim28

• Human Gene Id：
• 10155

• Human Swiss Prot No：
• Q13263

• Mouse Swiss Prot No：
• Q62318

• Immunogen：
• Purified recombinant human KAP1 / TIF1 beta protein fragments expressed in E.coli.

• Specificity：
• This antibody detects endogenous levels of KAP1 / TIF1 beta and does not cross-react with related proteins.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• wb 1:1000 icc 1:100

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• E3 SUMO protein ligase TRIM28;E3 SUMO-protein ligase TRIM28;FLJ29029;KAP 1;KAP-1;KRAB associated protein 1;KRAB interacting protein 1;KRAB-associated protein 1;KRAB-interacting protein 1;KRIP 1;KRIP-1;KRIP1;Nuclear corepressor KAP 1;Nuclear corepressor KAP-1;RING finger protein 96;RNF96;TF1B;TIF1 beta;TIF1-beta;TIF1B;TIF1B_HUMAN;Transcription intermediary factor 1 beta;Transcription intermediary factor 1-beta;TRIM28;Tripartite motif containing 28;tripartite motif containing protein 28;Tripartite motif-containing protein 28.

• Observed Band(KD)：
• 110kD

• Background：
• The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008],

• Function：
• domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,domain:The HP1 box is both necessary and sufficient for HP1 binding. The RING finger domain and the B-box domains mediate interaction with CEBPB. The PHD domain enhances the CEBPB transcriptional activity.,function:Forms a complex with a KRAB-domain transcription factor and increases the efficiency of KRAB-mediated repression. Silences transcription through an interaction with HP1 proteins. Acts as a corepressor of transcription for the KRAB zinc finger proteins and as a moderator of the repression activity. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of the Alpha-1-acid glycoprotein gene.,PTM:Phosphorylated upon DNA

• Subcellular Location：
• Nucleus . Associated with centromeric heterochromatin during cell differentiation through CBX1. .

• Expression：
• Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

LncRNA HCP5 enhances the proliferation and migration of cervical cancer via miR-216a-5p/CDC42 axis J Cancer. 2022; 13(6): 1882–1894. WB Human 1:2500

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