• Target：
• UBE1L

• Fields：
• >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways of neurodegeneration - multiple diseases

• Gene Name：
• UBA7

• Protein Name：
• Ubiquitin-like modifier-activating enzyme 7

• Human Gene Id：
• 7318

• Human Swiss Prot No：
• P41226

• Immunogen：
• Purified recombinant fragment of human UBE1L expressed in E. Coli.

• Specificity：
• UBE1L Monoclonal Antibody detects endogenous levels of UBE1L protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• UBA7;UBE1L;UBE2;Ubiquitin-like modifier-activating enzyme 7;Ubiquitin-activating enzyme 7;D8;Ubiquitin-activating enzyme E1 homolog

• Molecular Weight(Da)：
• 112kD

• References：
• 1. Cancer Res. 1992 Mar 15;52(6):1536-41
  2. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6071-5.
  3. Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3806-11.

• Background：
• The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008],

• Function：
• function:Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.,miscellaneous:There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.,tissue specificity:Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.,

• Subcellular Location：
• nucleus,nucleoplasm,cytosol,

• Expression：
• Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

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