• Target：
• MAP3K2

• Fields：
• >>MAPK signaling pathway;>>Gap junction;>>GnRH signaling pathway

• Gene Name：
• MAP3K2

• Protein Name：
• Mitogen-activated protein kinase kinase kinase 2

• Human Gene Id：
• 10746

• Human Swiss Prot No：
• Q9Y2U5

• Mouse Swiss Prot No：
• Q61083

• Immunogen：
• Purified recombinant fragment of human MEK Kinase-2 expressed in E. Coli.

• Specificity：
• MEK Kinase-2 Monoclonal Antibody detects endogenous levels of MEK Kinase-2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAP3K2;MAPKKK2;MEKK2;Mitogen-activated protein kinase kinase kinase 2;MAPK/ERK kinase kinase 2;MEK kinase 2;MEKK 2

• Molecular Weight(Da)：
• 70kD

• References：
• 1. Clin Cancer Res. 2009 Sep 1;15(17):5541-51.
  2. J Biol Chem. 2009 May 15;284(20):13533-41.

• Background：
• The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-524.,function:Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Upon EGF stimulation, translocates into the nucleus.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes.,

• Subcellular Location：
• Cytoplasm . Nucleus . Upon EGF stimulation, translocates into the nucleus.

• Expression：
• Brain,Platelet,T-cell,Teratocarcinoma,Whole embryo,

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs