• Target：
• Ku-80

• Fields：
• >>Non-homologous end-joining

• Gene Name：
• XRCC5

• Protein Name：
• X-ray repair cross-complementing protein 5

• Human Gene Id：
• 7520

• Human Swiss Prot No：
• P13010

• Mouse Gene Id：
• 22596

• Mouse Swiss Prot No：
• P27641

• Immunogen：
• Purified recombinant fragment of human Ku-80 expressed in E. Coli.

• Specificity：
• Ku-80 Monoclonal Antibody detects endogenous levels of Ku-80 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• XRCC5;G22P2;X-ray repair cross-complementing protein 5;86 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair pr

• Molecular Weight(Da)：
• 83kD

• References：
• 1. Breast Cancer Res. 2009;11(6):R83.
  2. Biochem Biophys Res Commun. 2009 Dec 18;390(3):738-42.

• Background：
• The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],

• Function：
• developmental stage:Expression increases during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86.,domain:The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of t

• Subcellular Location：
• Nucleus . Nucleus, nucleolus . Chromosome .

• Expression：
• Cervix carcinoma,Coronary artery,Heart,Neuroblastoma,Osteoblast,Thy

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