Histone H3 (Di Methyl Lys4) Polyclonal Antibody

    • Catalog No.:YH0004
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Histone H3
      • Fields:
      • >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus
      • Gene Name:
      • HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C
      • Protein Name:
      • Histone H3.1/Histone H3.2/Histone H3.3/Histone H3.3C
      • Immunogen:
      • Synthesized peptide derived from human Histone H3 around the di-methylation site of K4.
      • Specificity:
      • Di-Methyl-Histone H3 (K4) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when di-methylated at K4.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • H3K4ME2;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3.1;Histone H3.2;Histone H3/m;Histone H3/o;H3F3A;H3.3A;H3F3;PP781;H3F3B;H3.3B;Histone H3.3;H3F3C;Histone H3.3C;Histone H3.5
      • Observed Band(KD):
      • 17kD
      • Background:
      • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
      • Function:
      • caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l
      • Subcellular Location:
      • Nucleus. Chromosome.
      • Expression:
      • Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,
      • Products Images
      • Western Blot analysis of MCF-7 cells using Di-Methyl-Histone H3 (K5) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
      • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100