ZAP-70(PT2241) mouse mAb

    • 货号:YM6657
    • 应用:IHC-p,IF(paraffin section)
    • 种属:Human, Mouse
      • 基因名称:
      • ZAP70 SRK
      • 蛋白名称:
      • Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase)
      • Human Gene Id:
      • 7535
      • Human Swiss Prot No:
      • P43403
      • 免疫原:
      • Synthesized peptide derived from human ZAP-70
      • 特异性:
      • This antibody detects endogenous levels of human Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-chain associated protein) (Syk-related tyrosine kinase). Heat-induced epitope retrieval (HIER
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse/IgG1, Kappa
      • 稀释:
      • IHC-p 1:100-500,WB 1:500-2000
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -20°C/1 year
      • 背景:
      • zeta chain of T cell receptor associated protein kinase 70(ZAP70) Homo sapiens This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.,domain:The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.,function:Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.,online information:ZAP70 mutation db,PTM:Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 prote
      • 细胞定位:
      • Cytoplasmic, Nuclear
      • 组织表达:
      • Blood,Brain,Leukocyte,Lymphoid,T-cell,
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded human Tonsil. 1,ZAP-70 Antibody was diluted at 1:200(4° overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval
      • Immunohistochemical analysis of paraffin-embedded human Tonsil. 1,ZAP-70 Antibody was diluted at 1:200(4° overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval