ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

鼠兔五标六色荧光检测试剂盒

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Pentuple-Fluorescence kit (RS0038, Immunoway). CK-pan mouse mAb(YM6815 Immunoway) green, Ki-67 rabbit mAb(YM7002 Immunoway) red, Iba 1 mouse mAb(YM4765 Immunoway) purple,CD8 a mouse mAb(YM4815 Immunoway) cyan, CD20 mouse mAb(YM4814 Immunoway) yellow, The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Immunoway YS0004, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).