IRAK-1 (phospho Thr100) Polyclonal Antibody
- Catalog No.:YP0753
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- IRAK-1
- Fields:
- >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Toll-like receptor signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>Lipid and atherosclerosis
- Gene Name:
- IRAK1
- Protein Name:
- Interleukin-1 receptor-associated kinase 1
- Human Gene Id:
- 3654
- Human Swiss Prot No:
- P51617
- Mouse Gene Id:
- 16179
- Mouse Swiss Prot No:
- Q62406
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr100. AA range:66-115
- Specificity:
- Phospho-IRAK-1 (T100) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T100.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- IRAK1;IRAK;Interleukin-1 receptor-associated kinase 1;IRAK-1
- Observed Band(KD):
- 77kD
- Background:
- This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.,PTM:Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.,similarity:
- Subcellular Location:
- Cytoplasm . Nucleus . Lipid droplet . Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). .
- Expression:
- Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
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- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
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- July 13-2018
- Antibody-FAQs
- Products Images
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRAK1 (Phospho-Thr100) Antibody
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRAK1 (Phospho-Thr100) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from Jurkat cells treated with heat shock, using IRAK1 (Phospho-Thr100) Antibody. The lane on the right is blocked with the phospho peptide.