p47-phox (phospho Ser304) Polyclonal Antibody
- Catalog No.:YP0204
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Monkey
- Target:
- p47-phox
- Fields:
- >>Chemokine signaling pathway;>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
- Gene Name:
- NCF1
- Protein Name:
- Neutrophil cytosol factor 1
- Human Gene Id:
- 653361
- Human Swiss Prot No:
- P14598
- Mouse Swiss Prot No:
- Q09014
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser304. AA range:281-330
- Specificity:
- Phospho-p47-phox (S304) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S304.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3
- Observed Band(KD):
- 44kD
- Background:
- The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
- Function:
- disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,
- Subcellular Location:
- Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .
- Expression:
- Detected in peripheral blood monocytes and neutrophils (at protein level).
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is blocked with the phospho peptide.