• Target：
• CD148

• Fields：
• >>Adherens junction;>>Chemical carcinogenesis - reactive oxygen species

• Gene Name：
• PTPRJ

• Protein Name：
• Receptor-type tyrosine-protein phosphatase eta

• Human Gene Id：
• 5795

• Human Swiss Prot No：
• Q12913

• Mouse Gene Id：
• 19271

• Mouse Swiss Prot No：
• Q64455

• Immunogen：
• The antiserum was produced against synthesized peptide derived from the Internal region of human PTPRJ. AA range:861-910

• Specificity：
• CD148 Polyclonal Antibody detects endogenous levels of CD148 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PTPRJ;DEP1;Receptor-type tyrosine-protein phosphatase eta;Protein-tyrosine phosphatase eta;R-PTP-eta;Density-enhanced phosphatase 1;DEP-1;HPTP eta;Protein-tyrosine phosphatase receptor type J;R-PTP-J;CD148

• Observed Band(KD)：
• 150kD

• Background：
• The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms

• Function：
• catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,disease:Defects in PTPRJ are found in cancers of colon, lung, and breast.,function:May contribute to the mechanism of contact inhibition of cell growth.,PTM:N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,similarity:Contains 9 fibronectin type-III domains.,

• Subcellular Location：
• Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane . Cell junction. After T-cell stimulation, it is temporarily excluded from immunological synapses.

• Expression：
• Expressed in the promyelocytic cell line HL-60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the IL3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.

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