• Target：
• NM23-H1

• Fields：
• >>Purine metabolism;>>Pyrimidine metabolism;>>Drug metabolism - other enzymes;>>Metabolic pathways;>>Nucleotide metabolism;>>Biosynthesis of cofactors

• Gene Name：
• NME1

• Protein Name：
• Nucleoside diphosphate kinase A

• Human Gene Id：
• 4830

• Human Swiss Prot No：
• P15531

• Mouse Gene Id：
• 18102

• Mouse Swiss Prot No：
• P15532

• Rat Gene Id：
• 191575

• Rat Swiss Prot No：
• Q05982

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human NM23-H1. AA range:3-52

• Specificity：
• NM23-H1 Polyclonal Antibody detects endogenous levels of NM23-H1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• NME1;NDPKA;NM23;Nucleoside diphosphate kinase A;NDK A;NDP kinase A;Granzyme A-activated DNase;GAAD;Metastasis inhibition factor nm23;Tumor metastatic process-associated protein;nm23-H1

• Observed Band(KD)：
• 23kD

• Background：
• This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.,cofactor:Magnesium.,disease:This protein is found in reduced amount in tumor cells of high metastatic potential.,disease:This protein is found in reduced amount in tumor cells of high metastatic potential. Somatic mutations of NME1 are found in neuroblastoma. Increased NME1 in neuroblastoma is correlated with features of the disease that are associated with aggressive tumors. May therefore have distinct if not opposite roles in different tumors.,enzyme regulation:Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity.,function:Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the

• Subcellular Location：
• Cytoplasm . Nucleus . Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

• Expression：
• Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.

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