• Target：
• Nectin 4

• Fields：
• >>Adherens junction

• Gene Name：
• PVRL4

• Protein Name：
• Poliovirus receptor-related protein 4

• Human Gene Id：
• 81607

• Human Swiss Prot No：
• Q96NY8

• Mouse Swiss Prot No：
• Q8R007

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PVRL4. AA range:312-361

• Specificity：
• Nectin 4 Polyclonal Antibody detects endogenous levels of Nectin 4 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PVRL4;LNIR;PRR4;Poliovirus receptor-related protein 4;Ig superfamily receptor LNIR;Nectin-4

• Observed Band(KD)：
• 55kD

• Background：
• This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011],

• Function：
• function:Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells.,PTM:The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.,similarity:Belongs to the nectin family.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subcellular location:Colocalizes with MLLT4 at cadherin-based adherens junctions.,subcellular location:The secreted form is found in breast tumor patients.,subunit:Self-associates. Interacts via its Ig-like V-type domain with PVRL1/nectin-1 Ig-like V-type domain. Interacts via its C-terminus with MLLT4.,tissue specificity:Predominantly expressed in placenta. Not detecte

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted . The secreted form is found in breast tumor patients (PubMed:15784625).

• Expression：
• Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs