ACOT12 Polyclonal Antibody

    • Catalog No.:YT0086
    • Applications:IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • ACOT12
      • Fields:
      • >>Pyruvate metabolism;>>Metabolic pathways
      • Gene Name:
      • ACOT12
      • Protein Name:
      • Acyl-coenzyme A thioesterase 12
      • Human Swiss Prot No:
      • Q8WYK0
      • Mouse Swiss Prot No:
      • Q9DBK0
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330
      • Specificity:
      • ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • ACOT12;CACH;CACH1;STARD15;Acyl-coenzyme A thioesterase 12;Acyl-CoA thioesterase 12;Acyl-CoA thioester hydrolase 12;Cytoplasmic acetyl-CoA hydrolase 1;CACH-1;hCACH-1;START domain-containing protein 15;StARD15
      • Molecular Weight(Da):
      • 62kD
      • Background:
      • catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
      • Function:
      • catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
      • Subcellular Location:
      • Cytoplasm, cytosol .
      • Expression:
      • Chondrosarcoma Lung Metastasis,Liver,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.