Oct-1 (phospho Ser385) Polyclonal Antibody

    • Catalog No.:YP1021
    • Applications:IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • OCT1
      • Fields:
      • >>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis
      • Gene Name:
      • POU2F1
      • Protein Name:
      • POU domain class 2 transcription factor
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human POU2F1/OCT1 around the phosphorylation site of Ser385. AA range:361-410
      • Specificity:
      • Phospho-Oct-1 (S385) Polyclonal Antibody detects endogenous levels of 41183 protein only when phosphorylated at S385.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • POU2F1;OCT1;OTF1;POU domain; class 2, transcription factor 1;NF-A1;Octamer-binding protein 1;Oct-1;Octamer-binding transcription factor 1;OTF-1;POU2F2;OCT2;OTF2;POU domain, class 2, transcription factor 2;Lymphoid-restricted im
      • Molecular Weight(Da):
      • 76kD
      • Background:
      • The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010],
      • Function:
      • function:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.,PTM:Phosphorylated by PRKDC.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR, PGR and HCFC1.,tissue specificity:Ubiquitous. Isoform 2 is lymphocyte-specific.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Ubiquitous. Isoform 2 is lymphocyte-specific.
      • Products Images
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using POU2F1/OCT1 (Phospho-Ser385) Antibody
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).