Crk-L (phospho Tyr207) Polyclonal Antibody

    • Catalog No.：YP0869
    • Applications：WB;IHC;IF;ELISA
    • Reactivity：Human;Mouse;Rat;Monkey
      • Target：
      • Crk-L
      • Fields：
      • >>MAPK signaling pathway;>>ErbB signaling pathway;>>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Fc gamma R-mediated phagocytosis;>>Neurotrophin signaling pathway;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Renal cell carcinoma;>>Chronic myeloid leukemia
      • Gene Name：
      • CRKL
      • Protein Name：
      • Crk-like protein
      • Human Gene Id：
      • 1399
      • Human Swiss Prot No：
      • P46109
      • Mouse Swiss Prot No：
      • P47941
      • Immunogen：
      • The antiserum was produced against synthesized peptide derived from human CrkL around the phosphorylation site of Tyr207. AA range:173-222
      • Specificity：
      • Phospho-Crk-L (Y207) Polyclonal Antibody detects endogenous levels of Crk-L protein only when phosphorylated at Y207.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Polyclonal, Rabbit,IgG
      • Dilution：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • CRKL;Crk-like protein
      • Observed Band(KD)：
      • 39kD
      • Background：
      • This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009],
      • Function：
      • function:May mediate the transduction of intracellular signals.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4.,
      • Subcellular Location：
      • endosome,cytosol,cell-cell adherens junction,extracellular exosome,
      • Expression：
      • Skin,Spleen,
      • Products Images
      • Western Blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody
      • Immunofluorescence analysis of HUVEC cells, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using CrkL (Phospho-Tyr207) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from COS7 cells, using CrkL (Phospho-Tyr207) Antibody. The lane on the right is blocked with the phospho peptide.

      Attributes

      • Code：
      • YP0869
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 190 / 340 / 600
      • Delivery：
      • 2024-05-05
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