Crk II Monoclonal Antibody

    • Catalog No.：YM1024
    • Applications：WB
    • Reactivity：Human;Mouse;Dog;Rabbit
      • Target：
      • Crk II
      • Fields：
      • >>MAPK signaling pathway;>>ErbB signaling pathway;>>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Fc gamma R-mediated phagocytosis;>>Neurotrophin signaling pathway;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Renal cell carcinoma;>>Chronic myeloid leukemia
      • Gene Name：
      • CRK
      • Protein Name：
      • Adapter molecule crk
      • Human Gene Id：
      • 1398
      • Human Swiss Prot No：
      • P46108
      • Mouse Swiss Prot No：
      • Q64010
      • Immunogen：
      • Purified recombinant human Crk II protein fragments expressed in E.coli.
      • Specificity：
      • Crk II Monoclonal Antibody detects endogenous levels of Crk II protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Monoclonal, Mouse
      • Dilution：
      • WB 1:1000 - 1:2000. Not yet tested in other applications.
      • Purification：
      • Affinity purification
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • CRK;Adapter molecule crk;Proto-oncogene c-Crk;p38
      • Molecular Weight(Da)：
      • 34kD
      • Background：
      • This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],
      • Function：
      • domain:The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.,domain:The SH2 domain mediates interaction with SHB.,function:The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.,PTM:Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.,PTM:P
      • Subcellular Location：
      • Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .
      • Expression：
      • Embryonic lung,Epithelium,Eye,Lung,Placenta,
      • Products Images
      • Western Blot analysis using Crk II Monoclonal Antibody against LNCAP, A431, MCF7, HL1080 cell lysate.

      Attributes

      • Code：
      • YM1024
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 210 / 350 / 600
      • Delivery：
      • 2024-05-06
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