ATP-citrate synthase Monoclonal Antibody

    • Catalog No.：YM1013
    • Applications：WB;IF;FCM
    • Reactivity：Human;Mouse;Rat;Bovine;Chicken;Pig;sheep
      • Target：
      • ATP-citrate synthase
      • Fields：
      • >>Citrate cycle (TCA cycle);>>Metabolic pathways
      • Gene Name：
      • ACLY
      • Protein Name：
      • ATP-citrate synthase
      • Human Gene Id：
      • 47
      • Human Swiss Prot No：
      • P53396
      • Mouse Swiss Prot No：
      • Q91V92
      • Immunogen：
      • Purified recombinant human ATP-citrate synthase (C-terminus) protein fragments expressed in E.coli.
      • Specificity：
      • ATP-citrate synthase Monoclonal Antibody detects endogenous levels of ATP-citrate synthase protein.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Monoclonal, Mouse
      • Dilution：
      • WB 1:1000 - 1:2000. IF 1:100 - 1:500. Flow cytometry: 1:100 - 1:200. Not yet tested in other applications.
      • Purification：
      • Affinity purification
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • ACLY;ATP-citrate synthase;ATP-citrate;pro-S-)-lyase;ACL;Citrate cleavage enzyme
      • Molecular Weight(Da)：
      • 121kD
      • Background：
      • ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],
      • Function：
      • catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,
      • Subcellular Location：
      • Cytoplasm, cytosol .
      • Expression：
      • Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,
      • Products Images
      • Western Blot analysis using ATP-citrate synthase Monoclonal Antibody against K562, HeLa cell lysate.
      • Immunofluorescence analysis of HeLa cells using ATP-citrate synthase Monoclonal Antibody.
      • Flow cytometric analysis of HeLa cells stained with ATP-citrate synthase Monoclonal Antibody (red), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

      Attributes

      • Code：
      • YM1013
      • Size
      • 50μL / 100μL / 200μL
      • Price$：
      • 210 / 350 / 600
      • Delivery：
      • 2024-05-05
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