• Target：
• Pirh2

• Fields：
• >>p53 signaling pathway;>>Ubiquitin mediated proteolysis;>>Measles

• Gene Name：
• RCHY1

• Protein Name：
• RING finger and CHY zinc finger domain-containing protein 1

• Human Gene Id：
• 25898

• Human Swiss Prot No：
• Q96PM5

• Mouse Swiss Prot No：
• Q9CR50

• Immunogen：
• Purified recombinant fragment of human Pirh2 expressed in E. Coli.

• Specificity：
• Pirh2 Monoclonal Antibody detects endogenous levels of Pirh2 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RCHY1;ARNIP;CHIMP;PIRH2;RNF199;ZNF363;RING finger and CHY zinc finger domain-containing protein 1;Androgen receptor N-terminal-interacting protein;CH-rich-interacting match with PLAG1;E3 ubiquitin-protein ligase Pirh2;RING finger

• Molecular Weight(Da)：
• 30kD

• References：
• 1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50.
  2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21.
  3. Exp Cell Res. 2006 Oct 15;312(17):3370-8.
  4. Cell. 2003 Mar 21;112(6):779-91

• Background：
• The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013],

• Function：
• similarity:Contains 1 CHY-type zinc finger.,similarity:Contains 1 CTCHY-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with AR, PLAG1 and SCYL1BP1/NTKLBP1.,

• Subcellular Location：
• Nucleus. Nucleus speckle. Cytoplasm.

• Expression：
• Liver,Platelet,Skin,Uterus,

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