• Target：
• Mcl-1

• Fields：
• >>PI3K-Akt signaling pathway;>>Apoptosis;>>JAK-STAT signaling pathway;>>MicroRNAs in cancer

• Gene Name：
• MCL1

• Protein Name：
• Induced myeloid leukemia cell differentiation protein Mcl-1

• Human Gene Id：
• 4170

• Human Swiss Prot No：
• Q07820

• Mouse Swiss Prot No：
• P97287

• Immunogen：
• Purified recombinant fragment of human MCL-1 expressed in E. Coli.

• Specificity：
• Mcl-1 Monoclonal Antibody detects endogenous levels of Mcl-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MCL1;BCL2L3;Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT

• Observed Band(KD)：
• About 40kd in human,39kd in mouse and rat

• References：
• 1. Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269.
  2. Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251.

• Background：
• This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],

• Function：
• function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs

• Subcellular Location：
• Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.

• Expression：
• Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th

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