• Target：
• KLHL12

• Gene Name：
• KLHL12

• Protein Name：
• Kelch-like protein 12

• Human Gene Id：
• 59349

• Human Swiss Prot No：
• Q53G59

• Mouse Swiss Prot No：
• Q8BZM0

• Immunogen：
• Purified recombinant fragment of human KLHL12 expressed in E. Coli.

• Specificity：
• KLHL12 Monoclonal Antibody detects endogenous levels of KLHL12 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• KLHL12;C3IP1;Kelch-like protein 12;CUL3-interacting protein 1;DKIR homolog;hDKIR

• Molecular Weight(Da)：
• 63kD

• References：
• 1. Exp Cell Res. 2004 Oct 15;300(1):72-83.
  2. Gene. 1994 Jan 28;138(1-2):171-4.

• Background：
• This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],

• Function：
• domain:The BTB domain is required for interaction with CUL3.,function:Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.,tissue specificity:Highly expressed in testis and at lower levels in the submandibular salivary gland.,

• Subcellular Location：
• Cytoplasmic vesicle, COPII-coated vesicle .

• Expression：
• Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.

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