E2A Monoclonal Antibody

    • Catalog No.:YM0204
    • Applications:WB,FCM,ELISA
    • Reactivity:Human
      • Gene Name:
      • TCF3
      • Protein Name:
      • Transcription factor E2-alpha
      • Human Gene Id:
      • 6929
      • Human Swiss Prot No:
      • P15923
      • Mouse Swiss Prot No:
      • P15806
      • Immunogen:
      • Purified recombinant fragment of human E2A expressed in E. Coli.
      • Specificity:
      • E2A Monoclonal Antibody detects endogenous levels of E2A protein.
      • Formulation:
      • Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
      • Source:
      • Mouse
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
      • Purification:
      • Affinity purification
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • TCF3; BHLHB21; E2A; ITF1; Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription facto
      • References:
      • 1.J Biol Chem. 2000 Oct 27;275(43):33567-73.
        2.Mol Cell Biol. 2001 Mar;21(5):1866-73.
        3.J Biol Chem. 2003 Jan 24;278(4):2370-6. Epub 2002 Nov 14.
      • Background:
      • transcription factor 3(TCF3) Homo sapiens This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have bee
      • Function:
      • disease:Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1;19)(q23;p13.3) with PBX1; Translocation t(17;19)(q22;p13.3) with HLF. Inversion inv(19)(p13;q13) with TFPT.,function:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.,PTM:Phosphorylated following NGF stimulation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with GRIPE and FIGLA (By similarity). Interacts with PTF1A and TGFB1I1.
      • Subcellular Location:
      • nuclear chromatin,nucleus,nucleoplasm,transcription factor complex,cytoplasm,protein complex,RNA polymerase II transcription factor complex,
      • Expression:
      • Lymphoma,Muscle,PCR rescued clones,
      • Products Images
      • Western Blot analysis using E2A Monoclonal Antibody against A549 (1), A431 (2), HeLa (3), PANC-1 (4) and PC-3 (5) cell lysate.
      • Flow cytometric analysis of A549 cells using E2A Monoclonal Antibody (green) and negative control (purple).