RhoBTB1/2 Polyclonal Antibody

    • Catalog No.:YT4084
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • RhoBTB1/2
      • Fields:
      • >>Ubiquitin mediated proteolysis
      • Gene Name:
      • RHOBTB1/RHOBTB2
      • Protein Name:
      • Rho-related BTB domain-containing protein 1/2
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human RHBT2. AA range:61-110
      • Specificity:
      • RhoBTB1/2 Polyclonal Antibody detects endogenous levels of RhoBTB1/2 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • RHOBTB1;KIAA0740;Rho-related BTB domain-containing protein 1;RHOBTB2;DBC2;KIAA0717;Rho-related BTB domain-containing protein 2;Deleted in breast cancer 2 gene protein;p83
      • Observed Band(KD):
      • 83kD
      • Background:
      • The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008],
      • Function:
      • similarity:Belongs to the small GTPase superfamily. Rho family.,similarity:Contains 2 BTB (POZ) domains.,tissue specificity:Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.,
      • Subcellular Location:
      • intracellular,cytosol,plasma membrane,
      • Expression:
      • Ubiquitous, with highest levels in skeletal muscle, placenta, testis, stomach, and kidney, followed by uterus and adrenal gland. Expressed in a variety of fetal tissues.
      • Products Images
      • Western Blot analysis of various cells using RhoBTB1/2 Polyclonal Antibody
      • Western blot analysis of lysates from HepG2 and HeLa cells, using RHBT2 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).