A20 Polyclonal Antibody
- Catalog No.:YT0037
- Applications:WB;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- A20
- Fields:
- >>NF-kappa B signaling pathway;>>Necroptosis;>>NOD-like receptor signaling pathway;>>IL-17 signaling pathway;>>TNF signaling pathway;>>Measles;>>Epstein-Barr virus infection
- Gene Name:
- TNFAIP3
- Protein Name:
- Tumor necrosis factor alpha-induced protein 3
- Human Gene Id:
- 7128
- Human Swiss Prot No:
- P21580
- Mouse Gene Id:
- 21929
- Mouse Swiss Prot No:
- Q60769
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human TNAP3. AA range:321-370
- Specificity:
- A20 Polyclonal Antibody detects endogenous levels of A20 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- TNFAIP3;OTUD7C;Tumor necrosis factor alpha-induced protein 3;TNF alpha-induced protein 3;OTU domain-containing protein 7C;Putative DNA-binding protein A20;Zinc finger protein A20
- Observed Band(KD):
- 89kD
- Background:
- This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012],
- Function:
- function:Interacts with NAF1 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system and may contribute to the in vivo effects of TNF (By similarity). Has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains.,induction:By TNF-alpha.,similarity:Belongs to the peptidase C64 family.,similarity:Contains 1 OTU domain.,similarity:Contains 7 A20-type zinc fingers.,subunit:Homodimer. Interacts with NAF1, TAX1BP1 and TRAF2.,
- Subcellular Location:
- Cytoplasm. Nucleus. Lysosome.; [A20p50]: Cytoplasm.
- Expression:
- Epithelium,Testis,Trachea,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using A20 Polyclonal Antibody
- Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from RAW264.7 cells, using TNAP3 Antibody. The lane on the right is blocked with the synthesized peptide.