MAP-4 (phospho Ser696) Polyclonal Antibody
- Catalog No.:YP1158
- Applications:IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- MAP4
- Gene Name:
- MAP4
- Protein Name:
- Microtubule-associated protein 4
- Human Gene Id:
- 4134
- Human Swiss Prot No:
- P27816
- Mouse Gene Id:
- 17758
- Mouse Swiss Prot No:
- P27546
- Rat Gene Id:
- 367171
- Rat Swiss Prot No:
- Q5M7W5
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human MAP4 around the phosphorylation site of Ser696. AA range:662-711
- Specificity:
- Phospho-MAP-4 (S696) Polyclonal Antibody detects endogenous levels of MAP-4 protein only when phosphorylated at S696.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- MAP4;Microtubule-associated protein 4;MAP-4
- Molecular Weight(Da):
- 121kD
- Observed Band(KD):
- 121kD
- Background:
- The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
- Function:
- alternative products:Additional isoforms seem to exist,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Non-neuronal microtubule-associated protein. Promotes microtubule assembly.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.,similarity:Contains 3 Tau/MAP repeats.,similarity:Contains 4 Tau/MAP repeats.,
- Subcellular Location:
- Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center . Recruitment to microtubule is inhibited by microtubules polyglutamylation. .
- Expression:
- Bone marrow,Brain,Cerebellum,Colon carcinoma,Epithelium,Eye,Heart,Liver,Lym
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- Products Images
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.