CREB-2 (phospho Ser219) Polyclonal Antibody
- Catalog No.:YP0503
- Applications:WB;ELISA
- Reactivity:Human;Mouse
- Gene Name:
- ATF4
- Protein Name:
- Cyclic AMP-dependent transcription factor ATF-4
- Human Gene Id:
- 468
- Human Swiss Prot No:
- P18848
- Mouse Gene Id:
- 11911
- Mouse Swiss Prot No:
- Q06507
- Immunogen:
- Synthesized phospho-peptide around the phosphorylation site of human CREB-2 (phospho Ser219)
- Specificity:
- Phospho-CREB-2 (S219) Polyclonal Antibody detects endogenous levels of CREB-2 protein only when phosphorylated at S219.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- ATF4;CREB2;TXREB;Cyclic AMP-dependent transcription factor ATF-4;cAMP-dependent transcription factor ATF-4;Activating transcription factor 4;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding prot
- Observed Band(KD):
- 38kD
- Background:
- activating transcription factor 4(ATF4) Homo sapiens This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provid
- Function:
- function:Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.,similarity:Belongs to the bZIP family.,similarity:Contains 1 bZIP domain.,subcellular location:Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.,subunit:Interacts with the C-terminal region of GABBR1 via the leucine zipper of its C-terminal bZIP domain. Interacts with the C-terminal region of GABBR2 (By similarity). Binds DNA as a homo-or heterodimer. Interacts with the N-terminal region of CEP290.,
- Subcellular Location:
- Nucleus . Nucleus speckle . Cytoplasm . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Colocalizes with NEK6 at the centrosome (PubMed:20873783). Recruited to nuclear speckles following interaction with EP300/p300 (PubMed:16219772). .
- Expression:
- Bladder,Colon,Fibroblast,Leukemic T-cell,Lung,Ovary,Placenta,
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- Products Images
- Western Blot analysis of CoLo cells using Phospho-CREB-2 (S219) Polyclonal Antibody