• Target：
• Pim-1

• Fields：
• >>JAK-STAT signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Pathways in cancer;>>MicroRNAs in cancer;>>Acute myeloid leukemia

• Gene Name：
• PIM1

• Protein Name：
• Serine/threonine-protein kinase pim-1

• Human Gene Id：
• 5292

• Human Swiss Prot No：
• P11309

• Mouse Swiss Prot No：
• P06803

• Rat Gene Id：
• 24649

• Rat Swiss Prot No：
• P26794

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human Pim-1. AA range:281-330

• Specificity：
• Pim-1 Polyclonal Antibody detects endogenous levels of Pim-1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PIM1;Serine/threonine-protein kinase pim-1

• Observed Band(KD)：
• 50kD

• Background：
• The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Manganese.,function:Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.,induction:Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines.,PTM:Autophosphorylated on both serine/threonine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.,tissue specificity:Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 an

• Subcellular Location：
• [Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cell membrane.

• Expression：
• Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

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