• Target：
• PERK

• Fields：
• >>Mitophagy - animal;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis

• Gene Name：
• EIF2AK3

• Protein Name：
• Eukaryotic translation initiation factor 2-alpha kinase 3

• Human Gene Id：
• 9451

• Human Swiss Prot No：
• Q9NZJ5

• Mouse Swiss Prot No：
• Q9Z2B5

• Immunogen：
• Purified recombinant fragment of human PERK expressed in E. Coli.

• Specificity：
• PERK Monoclonal Antibody detects endogenous levels of PERK protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EIF2AK3;PEK;PERK;Eukaryotic translation initiation factor 2-alpha kinase 3;PRKR-like endoplasmic reticulum kinase;Pancreatic eIF2-alpha kinase;HsPEK

• Molecular Weight(Da)：
• 125kD

• References：
• 1. Autophagy. 2008 Apr 1;4(3):364-7.
  2. J Biol Chem. 2008 Jun 20;283(25):17020-9.
  3. Hum Mol Genet. 2008 Oct 15;17(20):3254-62.

• Background：
• The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act

• Subcellular Location：
• Endoplasmic reticulum membrane; Single-pass type I membrane protein.

• Expression：
• Ubiquitous. A high level expression is seen in secretory tissues.

Cheng, Jinbo, et al. "Vitamin D combined with resveratrol prevents cognitive decline in SAMP8 mice." Current Alzheimer Research 14.8 (2017): 820-833.

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